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miltenyi il 10 secretion assay  (Miltenyi Biotec)


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    Miltenyi Biotec miltenyi il 10 secretion assay
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Miltenyi Il 10 Secretion Assay, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+10+secretion+detection+kit/IL-10+Secretion+Assay+-+Detection+Kit+(PE)%2C+human/bio_rxiv__64898__2026__05__05__723043-198-9-9
    Average 97 stars, based on 5 article reviews
    miltenyi il 10 secretion assay - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging"

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging

    Journal: bioRxiv

    doi: 10.64898/2026.05.05.723043

    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Figure Legend Snippet: A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Techniques Used: Staining, Expressing, Activation Assay, Quantitative Proteomics, Immunofluorescence

    A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Figure Legend Snippet: A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Techniques Used: Expressing, Gene Expression, Activity Assay, Comparison

    Related Articles

    Staining:

    Article Title: The Development and Function of Regulatory B Cells Expressing IL-10 (B10 cells) Requires Antigen Receptor Diversity and TLR Signals
    Article Snippet: .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, CA) with subsequent staining for CD19 expression before cell sorting into IL-10 + CD19 + and IL-10 − CD19 + populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, CA).

    Article Title: Regulatory B cells and their uses
    Article Snippet: 11.1.5 B Cell Cytokine Transcript Expression Analysis Purified spleen B cells were cultured for 5 hours with LPS+PMA+ionomycin (L+PI). .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, Calif.).

    Article Title: Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells
    Article Snippet: Culture supernatant fluid IL-10 concentrations for triplicate samples were quantified using IL-10 OptEIA ELISA kits (BD Biosciences) following the manufacturer's protocols. .. IL10 transcript expression In some experiments, IL-10-secreting blood B cells were identified after 4 hours of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotec) with subsequent staining for CD19 expression before cell sorting into IL-10 + CD19 + and IL-10 − CD19 + populations. ..

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. B Cell IL10 Transcript Expression In some experiments, IL-10-secreting blood B cells were identified after 4 h of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using Qiagen RNeasy spin columns (Qiagen Ltd., Crawley, UK).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, Calif.).

    Expressing:

    Article Title: The Development and Function of Regulatory B Cells Expressing IL-10 (B10 cells) Requires Antigen Receptor Diversity and TLR Signals
    Article Snippet: .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, CA) with subsequent staining for CD19 expression before cell sorting into IL-10 + CD19 + and IL-10 − CD19 + populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, CA).

    Article Title: Regulatory B cells and their uses
    Article Snippet: 11.1.5 B Cell Cytokine Transcript Expression Analysis Purified spleen B cells were cultured for 5 hours with LPS+PMA+ionomycin (L+PI). .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, Calif.).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. 6.1.9 Microarray Expression Profiling For microarray analysis, viable IL-10 secreting B cells were detected after 5 hours of LPS, PMA, and ionomycin stimulation using an IL-10 secretion detection kit (Miltenyi Biotech) before cell sorting. .. RNAs from purified B cell subsets were prepared as above and processed for use on Affymetrix Mouse Genome 430 2.0 GeneChips (Affymetrix, Santa Clara, Calif.).

    Article Title: Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells
    Article Snippet: Culture supernatant fluid IL-10 concentrations for triplicate samples were quantified using IL-10 OptEIA ELISA kits (BD Biosciences) following the manufacturer's protocols. .. IL10 transcript expression In some experiments, IL-10-secreting blood B cells were identified after 4 hours of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotec) with subsequent staining for CD19 expression before cell sorting into IL-10 + CD19 + and IL-10 − CD19 + populations. ..

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. B Cell IL10 Transcript Expression In some experiments, IL-10-secreting blood B cells were identified after 4 h of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using Qiagen RNeasy spin columns (Qiagen Ltd., Crawley, UK).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. 6.1.9 Microarray Expression Profiling For microarray analysis, viable IL-10 secreting B cells were detected after 5 hours of LPS, PMA, and ionomycin stimulation using an IL-10 secretion detection kit (Miltenyi Biotech) before cell sorting. .. RNAs from purified B cell subsets were prepared as above and processed for use on Affymetrix Mouse Genome 430 2.0 GeneChips (Affymetrix, Santa Clara, Calif.).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, Calif.).

    FACS:

    Article Title: The Development and Function of Regulatory B Cells Expressing IL-10 (B10 cells) Requires Antigen Receptor Diversity and TLR Signals
    Article Snippet: .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, CA) with subsequent staining for CD19 expression before cell sorting into IL-10 + CD19 + and IL-10 − CD19 + populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, CA).

    Article Title: Regulatory B cells and their uses
    Article Snippet: 11.1.5 B Cell Cytokine Transcript Expression Analysis Purified spleen B cells were cultured for 5 hours with LPS+PMA+ionomycin (L+PI). .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, Calif.).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. 6.1.9 Microarray Expression Profiling For microarray analysis, viable IL-10 secreting B cells were detected after 5 hours of LPS, PMA, and ionomycin stimulation using an IL-10 secretion detection kit (Miltenyi Biotech) before cell sorting. .. RNAs from purified B cell subsets were prepared as above and processed for use on Affymetrix Mouse Genome 430 2.0 GeneChips (Affymetrix, Santa Clara, Calif.).

    Article Title: Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells
    Article Snippet: Culture supernatant fluid IL-10 concentrations for triplicate samples were quantified using IL-10 OptEIA ELISA kits (BD Biosciences) following the manufacturer's protocols. .. IL10 transcript expression In some experiments, IL-10-secreting blood B cells were identified after 4 hours of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotec) with subsequent staining for CD19 expression before cell sorting into IL-10 + CD19 + and IL-10 − CD19 + populations. ..

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. B Cell IL10 Transcript Expression In some experiments, IL-10-secreting blood B cells were identified after 4 h of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using Qiagen RNeasy spin columns (Qiagen Ltd., Crawley, UK).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. 6.1.9 Microarray Expression Profiling For microarray analysis, viable IL-10 secreting B cells were detected after 5 hours of LPS, PMA, and ionomycin stimulation using an IL-10 secretion detection kit (Miltenyi Biotech) before cell sorting. .. RNAs from purified B cell subsets were prepared as above and processed for use on Affymetrix Mouse Genome 430 2.0 GeneChips (Affymetrix, Santa Clara, Calif.).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. IL-10-secreting spleen B cells were identified using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using TRIzol (Invitrogen-Molecular Probes), with relative cytokine transcripts quantified by GeneChip analysis (Affymetrix Mouse Genome 430 2.0 GeneChips; Affymetrix, Santa Clara, Calif.).

    Microarray:

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. 6.1.9 Microarray Expression Profiling For microarray analysis, viable IL-10 secreting B cells were detected after 5 hours of LPS, PMA, and ionomycin stimulation using an IL-10 secretion detection kit (Miltenyi Biotech) before cell sorting. .. RNAs from purified B cell subsets were prepared as above and processed for use on Affymetrix Mouse Genome 430 2.0 GeneChips (Affymetrix, Santa Clara, Calif.).

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. 6.1.9 Microarray Expression Profiling For microarray analysis, viable IL-10 secreting B cells were detected after 5 hours of LPS, PMA, and ionomycin stimulation using an IL-10 secretion detection kit (Miltenyi Biotech) before cell sorting. .. RNAs from purified B cell subsets were prepared as above and processed for use on Affymetrix Mouse Genome 430 2.0 GeneChips (Affymetrix, Santa Clara, Calif.).

    In Vitro:

    Article Title: Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells
    Article Snippet: Culture supernatant fluid IL-10 concentrations for triplicate samples were quantified using IL-10 OptEIA ELISA kits (BD Biosciences) following the manufacturer's protocols. .. IL10 transcript expression In some experiments, IL-10-secreting blood B cells were identified after 4 hours of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotec) with subsequent staining for CD19 expression before cell sorting into IL-10 + CD19 + and IL-10 − CD19 + populations. ..

    Article Title: Regulatory B cells and their uses
    Article Snippet: .. B Cell IL10 Transcript Expression In some experiments, IL-10-secreting blood B cells were identified after 4 h of in vitro stimulation using an IL-10 secretion detection kit (Miltenyi Biotech, Auburn, Calif.) with subsequent staining for CD19 expression before cell sorting into IL-10+CD19+ and IL-10−CD19+ populations. .. Total RNA was extracted from the purified B cells using Qiagen RNeasy spin columns (Qiagen Ltd., Crawley, UK).



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    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
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    97
    Miltenyi Biotec il 10 secretion assay detection kit
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Il 10 Secretion Assay Detection Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    il 10 secretion assay  (Miltenyi Biotec)
    97
    Miltenyi Biotec il 10 secretion assay
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Il 10 Secretion Assay, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+10+secretion+detection+kit/IL-10+Secretion+Assay+-+Detection+Kit+(APC)%2C+human/pmc12302006-138-0-4
    Average 97 stars, based on 1 article reviews
    il 10 secretion assay - by Bioz Stars, 2026-09
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    		  Buy from Supplier
    il 10 secretion assay miltenyi biotec  (Miltenyi Biotec)
    97
    Miltenyi Biotec il 10 secretion assay miltenyi biotec
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Il 10 Secretion Assay Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+10+secretion+detection+kit/IL-10+Secretion+Assay+-+Detection+Kit+(APC)%2C+human/pm40449486-192-25-28
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    miltenyi 130 090 761 flowjo  (Miltenyi Biotec)
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    Miltenyi Biotec miltenyi 130 090 761 flowjo
    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for <t>surface</t> <t>IL-10</t> protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .
    Miltenyi 130 090 761 Flowjo, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Journal: bioRxiv

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging

    doi: 10.64898/2026.05.05.723043

    Figure Lengend Snippet: A, Schematic of Treg experimental design. Briefly, individual Tregs in CCEs were activated with T cell TransAct and stimulated with various cytokine cocktails for 6 days (Conditions I-IV) or 13 days (Conditions I+III). Tregs stimulated for 13 days were also stained for surface IL-10 protein levels. B , Uniform manifold approximation and projection (UMAP) of batch-corrected transcriptomic profiles of all 21,922 T cells from both 6 and 13 day time points colored by (left to right, top to bottom) FOXP3 expression, CellTypist predicted cell types from Hao et al. PBMC atlas (see methods) , experimental stimulation condition, and Leiden cluster. C , Composition plot of experimental condition by Leiden cluster for clusters 1-3. D , Dot plot of Treg activation (red) and suppression (blue) markers for Leiden clusters 1-3. E , Violin plot of tissue Treg scores of cells profiled at day 6 in each experimental condition (see Methods). F , Kernel density estimate plot for cells profiled at day 6 of cell area, and cell cycling score (see Methods). Dotted line represents delineation between low area and high area cells in G. G , Volcano plot of differential expression (DE) analysis between large Tregs (cell area > 3000 pixels, i.e. diameter > 15.4 μm), and small Tregs (cell area < 3000 pixels). Red points: genes upregulated in large cells (right, FDR < 0.05, log2FC > 1), or genes upregulated in small cells (left, FDR < 0.05, log2FC < -1). Shown right are two brightfield images of cells typifying these classes. H , Kernel density estimate (KDE) plots of IL-10 immunofluorescence (IF) signal for cells profiled at day 13 in majority Treg clusters, and cells in majority Tconv. clusters . The dotted line represents delineation between low IL-10 and high IL-10 cells in I. I , Volcano plot of DE analysis between Tconv. cells with high IL10 IF signal (IL-10 IF > 10) and low IL-10 IF signal (IL-10 IF < 10). Red points: genes upregulated in IL-10 IF-high Tconvs. (right, FDR < 0.05, log2FC > 1). Shown right IF images of two cells typifying these classes. J , DE analysis between T cells profiled on day 13 with high eccentricity (eccentricity > 0.9) and cells with low eccentricity (eccentricity < 0.9). Red points: genes upregulated in highly eccentric cells (right, FDR < 0.05, log2FC > 0.5), or genes upregulated in low eccentricity cells (left, FDR < 0.05, log2FC < -0.5). Shown right are two IF images of cells typifying these classes. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Article Snippet: On day 13, IL-10 secretion was assessed using the Miltenyi IL-10 Secretion Assay (Cat# 130-090-434).

    Techniques: Staining, Expressing, Activation Assay, Quantitative Proteomics, Immunofluorescence

    A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Journal: bioRxiv

    Article Title: Linking live-cell behavior to transcriptional responses across perturbations using dynamic caging

    doi: 10.64898/2026.05.05.723043

    Figure Lengend Snippet: A , Confidence scores of CellTypist predictions for each CD4 T class included in Hao et al. PBMC atlas (all cells included). B , T cell receptor (TCR), and inflammatory T reg signature scores for Leiden clusters 1-3 in (see methods). C , UMAP of 17,967 T cells profiled at 6 days colored by (left to right, top to bottom) experimental condition, CellTypist predicted cell type, tissue Treg score, cell size, and cycling score. D , UMAP of 3,955 T cells profiled at 13 days colored by (left to right) CellTypist predicted cell type, and IL-10 IF signal. E, UMAP of Hao et al. CD4 cells colored by (left to right) cell type, and IL10 expression. F, Dot plot of gene expression across cell types in Hao CD4 T cell atlas. G , Tr1 and Th2 signature scores for IL-10 IF high (IL-10 IF > 10) and IL-10 IF low (IL-10 IF < 10) T conventional cells (see methods). H , Inferred transcription factor activity score for IL-10 IF high and low Tconv cells. Transcription factor inference was performed using a univariate linear model from decoupler and the CollecTRI transcription factor database. I , UMAP of cells profiled at 13 days colored by (left to right) eccentricity, and Leiden cluster. J, Dot plot of gene expression for high (eccentricity > 0.5) and low eccentricity (eccentricity < 0.5) cells in each Leiden cluster in H. P values for gene score comparison and DE analysis were calculated using a two-sided Wilcoxon rank sum test. *Padj<0.05, **Padj<0.01, ***Padj<0.001 .

    Article Snippet: On day 13, IL-10 secretion was assessed using the Miltenyi IL-10 Secretion Assay (Cat# 130-090-434).

    Techniques: Expressing, Gene Expression, Activity Assay, Comparison